WebJan 4, 2007 · Example images of a FITC-tagged sample are shown in Figure 5. Here an image photographed using a Brightline Clinical FITC-LP01 filter set is compared to one taken using a commonly used soft-coated filter set. Case 2. Minimize Background and Maximize Brightness This case applies to sample slides that exhibit very high … WebFITC EGF p. Amc an m Straw Percp PE Texas Red 496.565 493 496 690 690 647 694 19 525 508 489 675 613 730'45 710150 670,30 610120 530130 530130 525150 780160 …
Fluorescein - Wikipedia
WebJan 10, 2024 · For example, Alexa Fluor®488, one of the most commonly used dyes, has an excitation maximum at 493 nm, which allows excitation with a standard 488 nm laser, and an emission maximum at 519 nm Alexa Fluor®488 is a fluorescein derivate and has similar properties than FITC. However, it shows better stability, brightness and lower pH … WebFluorochrome Guides. Cytek’s fluorochrome guides provide examples of many dyes full spectrum signatures along with stain indices, spread information, and other useful information. Download the fluorochrome guide that … lore olympus 213
BD Biosciences Relative Fluorochrome Brightness
WebAbstract. Fluorescein (resorcinolphthalein) is a synthetic organic photoactive dye compound, first synthesized by von Bayer in 1871 [1]. Some of the advantageous features of fluorescein fluorophores are good water solubility and absorption, emission, and maximum brightness at physiological pH (Anthoni et al., 1995) [2]. WebThe increased brightness of BV480 makes it a better choice for resolving dim markers. Spillover into channel filter BV421 BV605 BV650 BV711 BUV496 BUV563 BUV661 FITC Reagent 431/28* 450/40 610/20 660/20 710/50 515/30 562/40 670/30 530/30 Hu CD3 BV480 0% 13% 19% 5% 2% 12% 5% 1% 5% BV510 0% 6% 50% 18% 10% 21% 19% … WebFITC vs Alexa 488. This is the comparison of the brightness of the Alexa Fluor 488 and fluorescein conjugates that we publish, which indicates a closer to 1.5 to 2-fold advantage of the Alexa Fluor 488 dye fluorescence … lore olympus 215