WebJan 19, 2024 · To elucidate the molecular mechanism of METTL3 inhibition in lung cancer cells, we examined the effect of siRNA of METTL3 on the PI3K/AKT pathway of A549 … WebCellswerepretreated with/withoutTUDCA(100 μM)for3hoursbefore beingstimulated with TiPsforanother 24hours.Subsequently,thecellswerecollected andstainedwith theAnnexin …

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WebThe Annexin V-FITC/PI Apoptosis Kit was ordered from the 4A Biotech Company (Cat#FXP018-100, Beijing, China). The primary antibodies, including AKT CST … WebAug 4, 2024 · The Cell Cycle Analysis Kit (cat. no. FXP0311-100) and Annexin V/PI apoptosis kit (cat. no. FXP018-100; both Beijing 4A Biotech Co., Ltd.) were used to assess cell cycle distribution and apoptosis, respectively, per the manufacturer's instructions. Cell apoptosis was measured via PI/Annexin V-APC staining and analyzed via flow cytometry. nita faye flowers berryville

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WebJun 17, 2024 · Cells from different treatment groups were trypsinized without EDTA and resuspended with 1x binding buffer. The cells were stained with the annexin V/FITC … Webdays. Afterward, 0.2% Triton X-100 lysis buffer was used to lyses cells, and the optical density of the supernatant of the lysate was measured at 520 nm. The ALP activity was normalizing to the total protein content. Real-time quantitative PCR (qPCR) Cells were first treated with 0–400 μg/mL tanshinol for 48 h, and the total RNA in cells were Weband streptomycin (100 µg/ml) in a humidified atmosphere of 5% CO 2 at 37˚C. All the cells were confirmed to be free from ... FXP018-100) according to the manufacturer's instructions and analyzed by flow cytom-etry (FACS Calibur, BD Biosciences, San Jose, CA, USA). Statistical analyses. Unless stated otherwise, data are nit acronyms